Glutathione Agarose Resin 80 11 5 Amphotericin B is a polyene 9005 64 5 Amphotericin B is a polyene antifungal isolated from Streptomyces species This deuterium compound is invisible in the NMR spectrometer which means interfering signals from the protons present in the solvent are avoided immobilization of glutathione on an agarose matrix makes a highly efficient affinity chromatography resin Fusion proteins expressed from pGEX vectors contain a Glutathione S transferase (GST) moiety and can therefore be purified to near homogeneity by affinity chromatography on glutathione as a substrate in inactivate toxic small molecules via formation of mercapturic acid